10‑Gingerol exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells.
10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells.
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Abstract
Obesity is defined as excessive fat accumulation that can be detrimental to health and currently affects a large part of the global population. Obesity arises from excessive energy intake along with a sedentary lifestyle and leads to adipocytes with aggravated hypertrophy. Strategies have been designed to prevent and treat obesity. Nutrigenomics may serve a role in prevention of obesity using bioactive compounds present in certain foods with anti-obesogenic effects. Ginger (Roscoe) contains gingerols, key bioactive compounds that inhibit hypertrophy and hyperplasia of adipocytes. The present study aimed to evaluate the antiadipogenic activity of 10-gingerol (10-G) in the 3T3-L1 cell line. Three study groups were formed: Negative (3T3-L1 preadipocytes) and positive control (mature 3T3-L1 adipocytes) and 10-G (3T3-L1 preadipocytes stimulated with 10-G during adipogenic differentiation). Cell viability and lipid content were evaluated by MTT assay and Oil Red O staining, respectively. mRNA expression of CCAAT enhancer-binding proteinα(, peroxisome proliferator-activated receptorγ(, mechanistic target of rapamycin complex, sterol regulatory element binding transcription factor 1 (, acetyl-coenzyme A carboxylase (, fatty acid binding protein 4 (, and 18S rRNA (), was quantified by quantitative PCR. The protein expression of C/EPBαwas analyzed by western blot. In the 10-G group, lipid content was decreased by 28.83% (P
Affiliation
María Elizabeth Preciado-Ortiz
External References
- PubMed ID:
- 38025831
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