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Research Abstract Animal Study

Anti-obesity effects of water-dispersible turmeric extract via gut barrier and metabolite modulation in high-fat diet-fed mice.

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Abstract

Turmeric (Curcuma longa) exhibits anti-obesity properties, yet its low water solubility limits bioavailability. In this study, a water-dispersible turmeric rhizome extract (WDTE) was developed using nano-dispersion technology with maltodextrin as a wall material and characterized by UPLC-QTOF-MS, dynamic light scattering, and zeta potential analysis. The WDTE contained 10 identified metabolites, including five diarylheptanoids such as curcumin, demethoxycurcumin, and bisdemethoxycurcumin, with curcumin quantified at 7.3 % (w/w). WDTE displayed significantly improved colloidal characteristics compared to conventional extract, with reduced particle size (1095.0 ± 69.4 nm) and stable zeta potential (-41.77 ± 1.33 mV). In high-fat diet (HFD)-induced obese mice, oral administration of WDTE for 8 weeks reduced body weight gain, fat mass, and hepatic lipid accumulation. WDTE also normalized serum alanine transaminase and triglyceride levels, improved glucose tolerance, and restored insulin sensitivity. Mechanistically, WDTE suppressed the expression of key lipogenesis proteins (FAS, ACC, SREBP1, and PPARγ) and enhanced gut barrier function by upregulating tight junction proteins (ZO-1, occludin, and claudin-1), leading to reduced plasma endotoxin levels. Metabolomic profiling of fecal and serum samples revealed significant modulation in amino acid and bile acid pathways, notably including reduced levels of obesity-associated metabolites such as betaine and indole-3-propionic acid. These findings support WDTE as a multifunctional agent for managing obesity and metabolic endotoxemia through metabolic and intestinal modulation.

Affiliation

Yu Ra Lee

External References

PubMed ID:
40922178

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