Anti-persisters activity of Lacticaseibacillus rhamnosus culture filtrates against Pseudomonas aeruginosa.
Anti-Persisters Activity ofCulture Filtrates againstin Artificial Sputum Medium.
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Key Findings
Abstract
Persisters are antibiotic-tolerant bacteria, playing a role in the recalcitrance and relapse of many bacterial infections, includingpulmonary infections in Cystic Fibrosis (CF) patients. Among novel antimicrobial strategies, the use of probiotics and their products is emerging as a particularly promising approach. The aim of this study was to evaluate the anti-persisters activity of culture filtrate supernatants of(LRM-CFS) againstin artificial sputum medium (ASM), which resembles the CF lung environment. Planktonic persisters of two clinical strains of(PaCF1 and PaCF4) were obtained following two different procedures: (i) exposing stationary-phase cultures to cyanide m-chlorophenylhydrazone (CCCP) in LB medium; (ii) incubating stationary-phase cultures with high doses of tobramycin (128-fold MIC) in ASM. In addition, persisters from biofilm were obtained by exposing 48 h old biofilm ofto 128 x MIC of ciprofloxacin. LRM-CFS at dilutions of 1:6 and 1:4 resulted in being bactericidal in ASM against both PaCF1 and PaCF4 persisters obtained after CCCP or tobramycin treatment. Moreover, LRM-CFS at dilution 1:4 caused a reduction of antibiotic-tolerant bacteria in the biofilm of bothstrains. Overall, LRM-CFS represents a promising adjuvant therapeutic strategy againstrecalcitrant infections in CF patients.
Affiliation
Marta Bianchi
External References
- PubMed ID:
- 39000222
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