Antibacterial and anti-biofilm activity of kushenol N against methicillin-resistant Staphylococcus aureus.
AI Summary
Key Findings
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious clinical challenge due to antibiotic resistance and biofilm formation. Here, we demonstrate that kushenol N (KN), a natural flavonoid, exhibits potent antibacterial activity against Gram-positive bacteria, including multiple MRSA isolates (MICs of 8-32 μg/mL), and anti-biofilm activity against MRSA USA300. KN disrupted bacterial membranes and inhibited biofilm formation in a dose-dependent manner, reducing biomass by∼85 % at 1/2 MIC and lowering extracellular DNA to∼18 % of control. Key biofilm-associated genes (sarA, atl, agrA, clfA) were downregulated by 0.39-0.69-fold in MRSA USA300. KN displayed low cytotoxicity and minimal hemolytic activity (
Affiliation
Chunyan Dai
External References
- PubMed ID:
- 41187429
Comments
Sign in or create a free account to join the conversation.
Sign in to commentBe the first to comment.