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Research Abstract In Vitro Study

Antioxidant and antiproliferative activities of kale and wild cabbage

Antioxidant and Antiproliferative Activities of Kale (L. Var.DC.) and Wild Cabbage (Ten.) Polyphenolic Extracts.

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Abstract

Brassicaceae are rich in healthy phytochemicals that have a positive impact on human health. The aim of this study was to analyze the phenolic compounds and antioxidant and anticancer potential of traditional Croatian kale (L. var.DC.) and wild cabbage (Ten.) extracts. The phenolic groups and antioxidant activity were determined by spectrophotometry, selected phenolic compounds (ferulic acid, sinapic acid, salicylic acid, kaempferol, and quercetin) were analyzed by LC-MS/MS, and anticancer potential was evaluated in vitro using HeLa cells. The extracts of both plant species are rich in phenolic compounds and showed significant antioxidant activity at similar levels. LC-MS/MS detected sinapic acid as the most abundant phenolic acid, followed by ferulic acid, while salicylic acid was present at lower concentrations. A comparative analysis showed that wild cabbage contained significantly more sinapic acid, while kale contained more kaempferol and quercetin. Bothextracts at a concentration of 50µg mLshowed an antiproliferative effect on HeLa cells, while they did not affect the proliferation of normal human skin fibroblasts. Wild cabbage extract also showed an antiproliferative effect on HeLa cells at a lower applied concentration of 10µg mLof extracts. The clonogenic analysis also revealed the inhibitory effect of the extracts on HeLa colony growth.

Affiliation

Dario Lučić

External References

PubMed ID:
36838827

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