Apple cider vinegar exhibits promising antibiofilm activity against multidrug-resistant Bacillus cereus isolated from meat and their products.

Apple cider vinegar exhibits promising antibiofilm activity against multidrug-resistantisolated from meat and their products.

Rs Mahmoud, Afshin M Gharib, Norhan K Abd El-Aziz, E H A Ali, Abdul Halim Mokhtar, Gehan A Ibrahim, Achraf Ammar

Open Vet J

Jan 1, 2024

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Abstract

BACKGROUND: () biofilm is grown not only on medical devices but also on different substrata and is considered a potential hazard in the food industry. Quorum sensing plays a serious role in the synthesis of biofilm with its surrounding extracellular matrix enabling irreversible connection of the bacteria. AIM: The goal of the current investigation was to ascertain the prevalence, patterns of antimicrobial resistance, and capacity forbiofilm formation in meat and meat products in Egypt. METHODS: In all, 150 meat and meat product samples were used in this study. For additional bacteriological analysis, the samples were moved to the Bacteriology Laboratory. Thereafter, the antimicrobial, antiquorum sensing, and antibiofilm potential of apple cider vinegar (ACV) onwere evaluated. RESULTS: Out of 150 samples, 34 (22.67%) tested positive for. According to tests for antimicrobial susceptibility, everyisolates tested positive for colistin and ampicillin but negative for ciprofloxacin and imipenem. The ability to form biofilms was present in all 12 multidrug-resistantisolates (= 12); of these, 6 (50%), 3 (25%), and 3 (25%) isolates were weak, moderate, and strong biofilm producers, respectively. It is noteworthy that the ACV demonstrated significant inhibitory effects onisolates, with minimum inhibitory concentrations varying between 2 and 8μg/ml. Furthermore, after exposing biofilm-producingisolates to the minimum biofilm inhibitory concentrations 50 of 4μg/ml, it demonstrated good antibiofilm activity (>50% reduction of biofilm formation). Strong biofilm producers had down-regulated biofilm genes (and) and their regulator () compared to the control group, according to reverse transcriptase quantitative polymerase chain reaction analysis. CONCLUSION: Our study is the first report, that spotlights the ACV activity againstbiofilm and its consequence as a strong antibacterial and antibiofilm agent in the food industry and human health risk.