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Research Abstract Animal Study

Caffeic acid modulates protein disulfide isomerase-NLRP3 inflammasome signaling to mitigate inflammation in acute pneumonia.

Caffeic Acid Modulates Protein Disulfide Isomerase-NLRP3 Inflammasome Signaling to Mitigate Inflammation in Acute Pneumonia.

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Abstract

Caffeic acid (CA) is a polyphenol found in various of plants and daily beverages including coffee. It possesses diverse biological effects, including anti-inflammatory properties. Acute pneumonia represents a widespread inflammatory process; however, whether CA can mitigate acute pneumonia and its specific molecular mechanisms remain elusive. Here, we have demonstrated the robust anti-inflammatory effect of CA bothand. Additionally, protein disulfide isomerase (PDI) was identified as a potential target of CA via activity-based protein profiling strategy coupled with a chemical probe of CA. Moreover, CA was found to covalently bind to PDI through cysteine sites. Subsequentandexperiments further revealed the inhibition of PDI-mediated NLRP3 inflammasome signaling constituted the specific mechanism through which CA exerts anti-inflammatory effect. In conclusion, our study elucidates the molecular mechanisms underlying the amelioration of acute pneumonia by CA, providing valuable insights into its potential therapeutic application for inflammation-related diseases.

Affiliation

Guanjun Li

External References

PubMed ID:
41943849

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