Carbachol-induced phosphoinositide metabolism in slices of rat substantia nigra pars reticulata.
AI Summary
Key Findings
Abstract
Carbachol produced an approximately 2-fold stimulation, with respect to basal, of [3H]inositol monophosphate ([3H]IP1) accumulation in microdissected slices of rat substantia nigra pars reticulata (SNr), measured as a percentage of [3H]inositol incorporation. The response to carbachol was inhibited by 75 +/- 7% by 1 microM pirenzepine, consistent with a muscarinic M1 receptor-mediated response. Carbachol-induced [3H]IP1 accumulation was not reduced by pretreatment of the SNr slices with NMDA, in contrast to the 85% inhibition observed in striatal slices. The results suggest that measurement of carbachol-induced phosphoinositide hydrolysis may be a feasible approach for studying muscarinic M1 receptor function in slices of SNr.
Affiliation
Department of Pharmacology, University of Cambridge, UK.
External References
- PubMed ID:
- 8412567
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