Combining metabolomics and network pharmacology to clarify the mechanism of Angelica sinensis-Sophora flavescens pills in treating atopic dermatitis through the sweet-bitter harmonization theory.
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Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Rooted in "sweet-bitter harmonization" theory from Gu Jin Yi Jian (Vol. 9), Angelica sinensis-Sophora flavescens pills (ASP) have been traditionally used for inflammatory dermatoses. This study mechanistically validates ASP's efficacy against atopic dermatitis (AD) through calcium signaling and metabolic regulation. AIM OF THE STUDY: To elucidate ASP's anti-inflammatory mechanisms targeting AD pathophysiology, focusing on calcium-dependent macrophage polarization and metabolic reprogramming. MATERIALS AND METHODS: A network pharmacology approach identified 27 bioactive compounds and 176 AD-related therapeutic targets. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) metabolomics was applied to characterize key serum metabolic alterations in 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c AD mice treated with ASP at doses of 0.68-2.73 g/kg. Molecular docking analyses (AutoDock Vina) evaluated receptor-ligand binding affinities (threshold:ΔG
Affiliation
Peng Sun
External References
- PubMed ID:
- 40935216
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