Effect of curcumin on methotrexate-induced ovarian damage and follicle reserve.
Effect of curcumin on methotrexate-induced ovarian damage and follicle reserve in rats: the role of PARP-1 and P53.
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Abstract
BACKGROUND: Methotrexate (MTX) is an agent used in the treatment of many neoplastic and non-neoplastic diseases and is known to cause oxidative damage in normal tissues. Curcumin (Cur) is a natural polyphenol compound with powerful antioxidant and antiapoptotic effects. In this study we investigate the effects of Cur on MTX-induced ovarian damage. MATERIALS AND METHODS: Thirty-two young adult female Wistar albino rats were divided into four groups: (1) Control ( = 8): only vehicle group, (2) Cur ( = 8): Cur-only group (200 mg/kg/day), (3) MTX ( = 8): MTX-only group (0.35 mg/kg/day), (4) MTX+Cur ( = 8): The group was given MTX (0.35 mg/kg/day) and Cur (200 mg/kg/day) for 28 days. Then, SOD, CAT, MDA, AMH levels were measured using ELISA kits. Follicle count was performed on H&E stained slides. In addition, the expressions of P53 and PARP-1 were analysed by immunohistochemistry. RESULTS: MDA levels were seen to be higher in the MTX group than in the MTX+Cur group (
Affiliation
Mete Keçeci
External References
- PubMed ID:
- 39729361
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