Research Abstract
[Expression of a snake venom plasminogen activator TSV-PA in insect cells].
Cao Y, Yu GS, Yang SL, Gong Y
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Sep 1, 2001
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1 min read
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Abstract
A snake venom gene TSV-PA was inserted into the donor plasmids pFastBacHTa and expressed in Tn-5B1-4 cells. SDS-PAGE analysis revealed that the molecular weight of expressed product of TSV-PA were 33 kD. It was also proved by Western blot. The result of enzyme activity showed that TSV-PA protein expressed in insect cells had a higher activity.
Affiliation
Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
External References
- PubMed ID:
- 11797210
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