[Formation of polysomes in virus-specific RNA in the transcriptive complex of Sendai virus infected cells].

Abstract

Transcriptive complex in the cytoplasm of Sendai virus infected cells included parental RNA in the form of ribonucleoprotein, nascent RNA and polysomes. Its buoyant density in CsC1 was 1.45 g/ml. The complex dissociated three hours after the infection, and nascent RNA was fully separated from the parental RNP. The template for polysomes within the complex was identified under conditions when the complex was dissociated in cycloheximide-treated cells. Polysomes were revealed in the association with nascent RNA. They sedimented in preribosmal region of sucrose gradient, and had a buoyant density in CsC1 of 1.49 g/ml. Mild treatment with ribonuclease split the polysomes into monoribosomes.