G. lucidum induces apoptosis and impairs cancer cell proliferation through metabolic disruption and gene modulation.
Evaluation of Anticancer Potential ofon MCF-7 Breast Cancer Cells Through Genetic Transcription of Energy Metabolism.
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Abstract
has long been recognized for its medicinal properties, particularly due to its antioxidant, anti-inflammatory, and pro-apoptotic components such as polysaccharides and triterpenoids. This study aimed to evaluate the cytotoxic and molecular effects of ethanol and methanol extracts ofas well as doxorubicin on MCF-7 breast cancer cells. The cytotoxicity was assessed via MTT assay. The methanol extract showed stronger cytotoxicity (IC: 62.37µg/mL) than the ethanol extract, while doxorubicin exhibited an ICvalue of 0.66 mM. Phenolic profiling by HPLC revealed high levels of vanillic acid, gallic acid and (-)-epicatechin in the methanol extract, while volatile compounds such as hexanal and acetic acid were identified by GC-MS. Flow cytometric analysis demonstrated G0/G1 phase cell cycle arrest and an increase in early and late apoptotic populations. Gene expression studies using RT-qPCR showed significant downregulation of,, andkey regulators of energy metabolism and epigenetic modification. On the other hand, doxorubicin treatment upregulatedand, while a slight downregulation was observed in. These molecular changes suggest thatinduces apoptosis and impairs cancer cell proliferation through metabolic disruption and gene modulation.
Affiliation
Levent Gülüm
External References
- PubMed ID:
- 41462671
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