Growth inhibition and apoptotic effect of pine extract and abietic acid on MCF-7 breast cancer cells.
Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach.
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Key Findings
Abstract
In vitro anti-proliferative activity ofextract and its purified abietic acid was assessed against different human cancer cell lines (HepG-2, MCF-7 and HCT-116) compared to normal WI-38 cell line. Abietic acid showed more promising ICvalues against MCF-7 cells than pine extract (0.06µg/mL and 0.11 µM, respectively), with insignificant cytotoxicity toward normal fibroblast WI-38 cells. Abietic acid triggered both G/M cell arrest and subG-Gsubpopulation in MCF-7, compared to SubG-Gsubpopulation arrest only for the extract. It also induced overexpression of key apoptotic genes (and) and downregulation of both proliferation () and oncogenic (and) genes. Additionally, abietic acid induced overexpression of cytochrome-C protein. Furthermore, it increased levels of total antioxidants to diminish carcinogenesis and chemotherapy resistance.is a valuable source of active abietic acid, an antiproliferative agent to MCF-7 cells through induction of apoptosis with promising future anticancer agency in breast cancer therapy.
Affiliation
Hesham Haffez
External References
- PubMed ID:
- 35011526
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