Investigation of the effect of C-phycocyanin on sonic hedgehog pathway-related Gli1 and Bcl-2 gene expression in MKN45 gastric cancer cells.
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Abstract
Gastric cancer (GC) is the third leading cause of cancer-related mortality worldwide. Many traditional treatments for this type of cancer, including chemotherapy and surgery, have significant side effects that limit their effectiveness. Therefore, developing new therapeutic agents with fewer side effects and higher efficacy is crucial. C-Phycocyanin (C-PC), found in Spirulina platensis, exhibits antitumor properties; however, the molecular mechanisms underlying its action remain unclear. This study aims to determine the anti-cancer activity of C-PC extracted from Spirulina platensis on the MKN45 gastric cancer cell line and its impact on the expression of the Gli1 gene from the Sonic hedgehog (Shh) signaling pathway and Bcl-2 as a target gene of Gli1. This research was conducted as an interventional laboratory study at the Gene Fan Telma laboratory and North Research Center of the Pasteur Institute of Iran. The anti-cancer effects of C-PC extracted from Spirulina platensis were investigated. Cytotoxic and antiproliferative activities were evaluated using the MTT assay with various concentrations of C-PC and different incubation times. Real-time PCR was employed to assess the expression levels of Gli1 and Bcl-2 genes. The HF2FF cell line (normal human fibroblasts) was used as a control to evaluate C-PC's selectivity and safety profile. The results indicated that C-PC is a natural compound with therapeutic potential for various cancers, particularly gastric cancer. C-PC significantly affects the MKN45 gastric cancer cell line by reducing proliferation and inducing apoptosis by downregulating the anti-apoptotic gene Bcl-2. Furthermore, C-PC did not affect Bcl-2 expression in normal cells, underscoring its potential for cancer therapy use to minimize unwanted side effects. The results indicate that C-PC selectively triggers apoptosis in MKN45 gastric cancer cells and may enhance the treatment of gastric cancer by partially modifying the expression of anti-apoptotic genes. In conclusion, C-PC effectively inhibits the growth and proliferation of MKN45 gastric cancer cells while promoting apoptosis by downregulating Bcl-2 gene expression. Furthermore, C-PC demonstrates a protective role by increasing Bcl-2 expression in normal cells, thereby improving treatment efficacy and minimizing undesirable side effects. In conclusion, it can be inferred that C-PC may influence the proliferation rate of gastric cancer cell lines by partially altering anti-apoptotic gene expression.
Affiliation
Maryam Lotfi
External References
- PubMed ID:
- 40699394
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