Korean red ginseng aqueous extract improves markers of mucociliary clearance by stimulating chloride secretion.
Korean Red Ginseng aqueous extract improves markers of mucociliary clearance by stimulating chloride secretion.
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Key Findings
Abstract
Background: Abnormal chloride (Cl) transport has a detrimental impact on mucociliary clearance in both cystic fibrosis (CF) and non-CF chronic rhinosinusitis. Ginseng is a medicinal plant noted to have anti-inflammatory and antimicrobial properties. The present study aims to assess the capability of red ginseng aqueous extract (RGAE) to promote transepithelial Clsecretion in nasal epithelium. Methods: Primary murine nasal septal epithelial (MNSE) [wild-type (WT) and transgenic CFTR], fisher-rat-thyroid (FRT) cells expressing human WT CFTR, and TMEM16A-expressing human embryonic kidney cultures were utilized for the present experiments. Ciliary beat frequency (CBF) and airway surface liquid (ASL) depth measurements were performed using micro-optical coherence tomography (μOCT). Mechanisms underlying transepithelial Cltransport were determined using pharmacologic manipulation in Ussing chambers and whole-cell patch clamp analysis. Results: RGAE (at 30μg/mL of ginsenosides) significantly increased Cltransport [measured as change in short-circuit current (ΔI = μA/cm)] when compared with control in WT and CFTRMNSE (WT vs control = 49.8±2.6 vs 0.1+/-0.2, CFTR = 33.5±1.5 vs 0.2±0.3, p
Affiliation
Do-Yeon Cho
External References
- PubMed ID:
- 33437158
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