Lactiplantibacillus plantarum P101 ameliorates TiO2 NP-induced bone injury.
P101 Ameliorates TiONP-Induced Bone Injury in Young Rats by Remodeling the Gut Microbiota and Inhibiting the Production of Pro-Inflammatory Cytokines.
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Key Findings
Abstract
PURPOSE: To evaluate the therapeutic effect of oral administration ofP101 (P101) on skeletal injury in young rats exposed to titanium dioxide nanoparticles (TiONPs), and explore the potential mechanism. METHODS: Four-week-old male rats were orally administration to TiONPs and supplemented with P101 2 hours later for 4 weeks. The growth and development, food intake, bone metabolism and serum inflammatory markers of the rats were evaluated. Their tibias were observed and evaluated using microcomputed tomography (micro-CT), tartrate-resistant acid phosphatase (TRAP) staining, immunohistochemistry (IHC) and real-time quantitative PCR (RT-qPCR). We observed the tibia growth plate using safranin and fast green staining. 16S rDNA sequence analysis of fecal samples was performed to observe changes in the gut microbiota. RESULTS: Our results showed that TiONPs can lead to bone growth inhibition and osteoporosis, induce intestinal flora imbalance, and induce inflammation in young rats. Further mechanistic studies suggested that TiONPs disrupts intestinal flora and increases serum IL-1βlevels, which increased the expression of RANKL in bone, thereby enhancing osteoclast differentiation and function, leading to bone loss. Through a P101 supplementation experiment, we found that P101 ameliorated the inflammation and osteoporosis on bone caused by TiONPs. CONCLUSION: This study showed that the mechanism by which P101 alleviates bone damage caused by TiONPs may be through restoring intestinal microbial homeostasis and inhibiting inflammatory response.
Affiliation
Lixin Xie
External References
- PubMed ID:
- 39539967
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