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Research Abstract

Large-scale purification of hepatitis B surface antigen.

Vnek J, Prince AM
Journal of clinical microbiology
Jun 1, 1976
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Abstract

Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02 M sodium phosphate buffer.

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