Linking di-2-ethylhexyl phthalate (DEHP) exposure to osteoporosis through population analysis, network toxicology, and experimental evidence.
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Key Findings
Abstract
BACKGROUND: Osteoporosis is a major public health problem, and environmental toxicants such as di(2-ethylhexyl) phthalate (DEHP) have been implicated as potential risk factors. However, the molecular mechanisms linking DEHP exposure with osteogenic impairment remain unclear. METHODS: The association between DEHP exposure and osteoporosis risk using NHANES data, and employed network toxicology and molecular docking to identify key regulatory targets. To further verify these predictions, wet-lab experiments including Western blotting and quantitative PCR (qPCR) were conducted in osteoblast-related cells under control (CON), osteoporosis (OP), DEHP, and combined OP+DEHP conditions. RESULTS: Epidemiological analysis revealed that higher DEHP exposure was associated with increased osteoporosis risk. In the fully adjusted model, participants in the highest quartile of ln∑DEHP had higher odds of osteoporosis compared with those in the lowest quartile (OR = 1.32, 95% CI = 0.76-1.66, P for trend
Affiliation
Liji Li
External References
- PubMed ID:
- 41990860
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