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Research Abstract In Vitro Study

Myricetin attenuates lipopolysaccharide-induced acute lung injury.

Myricetin attenuates lipopolysaccharide-induced acute lung injury by suppressing pyroptosis via activation of the Nrf2/HO-1/NQO1 signaling pathway.

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Abstract

OBJECTIVE: To investigate the protective role and underlying mechanisms of Myricetin (Myr) in lipopolysaccharide (LPS)-induced acute lung injury (ALI), with emphasis on its modulation of oxidative stress and pyroptosis. METHODS: LPS-induced ALI in mice and an in vitro alveolar type II epithelial cell (AT2) model were employed to assess Myr's effects on lung pathology, oxidative stress, Nrf2/HO-1/NQO1 signaling, and NLRP3 inflammasome-associated pyroptosis. Evaluations included HE staining, lung wet-to-dry weight ratio, measurements of oxidative stress biomarkers (GSH, MDA, and SOD), Western blotting, ELISA, TUNEL assay, and related techniques. RESULTS: Myr markedly mitigated LPS-induced histopathological injuries in lung tissue (P

Affiliation

Jing Cao

External References

PubMed ID:
42144067

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