Pectin-type polysaccharides from raspberry (Rubus idaeus L.): structure characterization and activity against DSS-induced colitis.
AI Summary
Key Findings
Abstract
This study explored the structural properties and therapeutic potential of RAP-3 A, a pectin-type polysaccharide extracted from raspberry (Rubus idaeus L.), in DSS (dextran sodium sulfate)-induced colitis. Structural analysis revealed that RAP-3 A, with a molecular weight of 51.03 kDa, is primarily composed of a backbone of 1, 4-galacturonic acid glycan, incorporating a 1, 4-linkedα-GalpA and 1, 2-linkedα-Rhap disaccharide repeating unit, with branches at C-4 of the Rhap residue comprising 1, 4-linkedβ-Galp, 1, 6-linkedβ-Galp, or 1, 5-linkedα-Araf. Remarkably, RAP-3 A significantly alleviated colitis symptoms in mice, including weight loss, disease activity index (DAI), and histopathological damage. Mechanistically, RAP-3 A inhibited the TLR4/NF-κB pathway, enhanced intestinal barrier function by upregulating ZO-1 and occludin, and reduced goblet cell depletion. Furthermore, RAP-3 A promoted a favorable shift in gut microbiota composition, increasing the abundance of beneficial SCFA-producing bacteria such as Bacteroidales_S24-7, Rikenellaceae, Clostridiales, Oscillospira, and Lachnospiraceae while suppressing pathogenic bacteria, notably Escherichia. GC-MS analysis further confirmed that RAP-3 A significantly increased fecal SCFAs levels. Collectively, these findings suggest that RAP-3 A holds considerable potential for development as a functional food aimed at the treatment of colitis.
Affiliation
Zuman Dou
External References
- PubMed ID:
- 40484583
Comments
Sign in or create a free account to join the conversation.
Sign in to commentBe the first to comment.