Polymerase chain reaction approaches to Culicoides (Diptera: Ceratopogonidae) identification.
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Key Findings
Abstract
Molecular genetic approaches such as polymerase chain amplification (PCR) of target genomic sequences are finding wide application in systematic and taxonomic studies of arthropods. PCR-based techniques that preclude the need for target DNA sequence information of the species of interest facilitate molecular taxonomic studies. Two such techniques, tDNA-PCR (DNA encoding tRNAs is the analyte) and RAPD-PCR (randomly amplified polymorphic DNA) were investigated for their ability to differentiate certain North American Culicoides spp. larvae and adults.
Affiliation
Department of Microbiology, Colorado State University, Fort Collins 80523.
External References
- PubMed ID:
- 8433330
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