The potential alleviating property against NAFLD by Ganoderma lucidum with bacteria-enzyme synergistic fermentation.
The Potential Alleviating Property Against NAFLD byWith Bacteria-Enzyme Synergistic Fermentation.
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Abstract
is an edible and medicinal fungus. Triterpenoids, particularly ganoderic acids, represent the principal bioactive constituents in. Bacteria-enzyme synergistic fermentation broth of(FBG) was optimized as a potential alternative to traditional water-extracted(WEG). The optimized parameters withfermentation exhibited the highest content of ganoderic acid F (GAF). Administration of FBG led to a pronounced decline in lipid deposition and contents of intracellular total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), reactive oxygen species (ROS), and malonic dialdehyde (MDA). The FBG treatment also markedly increased the activities of high-density lipoprotein cholesterol (HDL-C), superoxide dismutase (SOD), and catalase (CAT). The results of network pharmacology analysis and molecular docking suggested that GAF exhibits a robust interaction with the core targets sodium leak channel, nonselective (NALCN) and eukaryotic translation elongation factor 1 alpha 2 (EEF1A2). Enzyme-linked immunosorbent assay (ELISA) results demonstrated that GAF could effectively reduce lipid accumulation while upregulating EEF1A2, phosphorylated AMP-activated protein kinase (p-AMPK), and peroxisome proliferator-activated receptor alpha (PPAR-α) protein expression. Collectively, these findings indicate that GAF alleviates free fatty acid (FFA)-induced hepatic lipid accumulation through activation of the EEF1A2/p-AMPK/PPAR-αsignaling pathway, suggesting a potential mechanism involving AMPK-related pathways.
Affiliation
Manhui Sun
External References
- PubMed ID:
- 42255694
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