Protective in vitro effects of Aloe vera on doxorubicin-induced ovarian toxicity in mice.
Protectiveeffects ofon doxorubicin-induced ovarian toxicity in mice.
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Abstract
The present study investigated the protective effect ofextract against the toxic effects of doxorubicin (DOX) on mice ovaries cultured in vitro. Femalemice with regular estrous cycle were used. The animals (n = 48) had their ovaries collected and cultured individually in a 24-well plate at 37.5°C in 5% COfor 6 days. The ovaries were culture DMEMalone or supplemented with DOX (0.3μg/ml), as well as both DOX and(5%, 10%, 25%, or 50%). After culture, ovaries were fixed for histological analysis (follicle morphology, growth and activation, extracellular matrix (ECM) configuration and stromal cell density), immunohistochemistry (expression) or stored at - 80°C to evaluate the levels of mRNA for superoxide dismutase (), catalase (), nuclear factor-erythroid 2-related factor 2 () and tumoral necrosis factor-α() by real-time PCR. The results showed that ovaries cultured with DOX had a lower percentage of normal follicles and reduced stromal cell density, but whenextract was added to the culture medium, there was a protective effect on the ovarian structure against the deleterious effects of DOX. In addition, ovaries cultured with both DOX and(10 and 25%) had reducedimmunostaining and increased expression of mRNA for antioxidant enzymes (,, and). In conclusion,is associated with a protective effect on ovarian follicles and stromal cells against DOX-induced toxicity. Therefore,has great potential to preserve fertility in patients undergoing chemotherapy treatment, which often cause irreversible damage to oocytes.
Affiliation
Ernando I T de Assis
External References
- PubMed ID:
- 40757498
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