Purified cucurbitacin D leads to alterations of apoptotic and autophagic genes expression in MDA-MB-468 and MCF-7 human breast cancer.
Purified Cucurbitacin D Leads to Alterations of Apoptotic and Autophagic Genes Expression in MDA-MB-468 and MCF-7 Human Breast Cancer Cells.
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Key Findings
Abstract
AIM: The use of plant-derived drugs in cancer therapy is widely considered in the treatment of different malignancies including breast cancer. Cucurbitacin D (CuD) is able to induce apoptosis in cancerous cells through different signaling pathways. The aim of this study was to examine the effect of different concentrations of CuD on viability and death pattern. METHODS: Antiproliferative effects of CuD on these cell lines' viability were investigated using the MTT assay. Real-time PCR was applied to evaluate the expression alterations of Bcl-2, Bax, caspase-3, p53 (that related to apoptotic cell death pathway), Atg5, Beclin-1, PTEN, and Akt genes (autophagy genes) in the MCF-7 (ER positive) and MDA-MB-468 (triple negative) breast cancer cells. RESULTS: Significant dose-dependent and antiproliferative effects of CuD were observed on MCF-7 and MDA-MB-468 cells after 24 h with IC50 value about 30 and 25µM, respectively (p
Affiliation
Elham Zeinali
External References
- PubMed ID:
- 40903794
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