Synergistic effects of Coptis rhizome and rhubarb in inhibiting MDA-MB-231 triple-negative breast cancer metastasis.
Synergistic effects of Coptis rhizome and rhubarb in inhibiting MDA-MB-231 triple-negative breast cancer metastasis: Potential involvement of coptisine and resveratroloside.
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Key Findings
Abstract
Metastasis, particularly in aggressive triple-negative breast cancer (TNBC), is the leading cause of breast cancer-related mortality. San-Huang-Xie-Xin-Tang (SHXXT), a heat-clearing herbal formula, is commonly prescribed by Chinese medicine physicians to treat breast cancer patients, and our previous study found that its treatment was associated with reduced mortality in these patients. To further explore its therapeutic potential, we investigated the combined effect of its key ingredients, Coptis rhizome and rhubarb, on the metastasis of TNBC by using MDA-MB-231 cell line. Data showed that the water extract of SHXXT reduced cell migration without inducing cell death, and oral administration of Coptis rhizome and rhubarb extracts significantly suppressed metastasis in vivo. Notably, this combination exhibited a potent synergistic inhibitory effect on cell migration and invasion, with a combination index of 0.2. Further analysis identified coptisine and resveratroloside as key active compounds that synergistically inhibit cell mobility. They modulate divergent epithelial-to-mesenchymal transition-related protein expressions, and this complementary regulation may underlie their synergistic effect. Taken together, our findings demonstrate that the water extracts of Coptis rhizome and rhubarb synergistically inhibit metastatic property of TNBC cells, with coptisine and resveratroloside contributing to this effect at least partly, suggesting their potential for the development of new adjuvant therapies for TNBC treatment.
Affiliation
Yun-Hao Dai
External References
- PubMed ID:
- 40744130
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