[The suppressive effect of HLA-DQw6 genes on collagen-induced arthritis in mice].
AI Summary
Key Findings
Abstract
We investigated the effect of HLA-DQw6 genes on collagen-induced arthritis (CIA) in mice, based on our previous observation that the frequency of HLA-DR4-Dw15-DQw4 haplotype was increased in the patients with rheumatoid arthritis (RA) in the Japanese whereas the frequency of HLA-DR2-Dw12-DQw6 was decreased in them significantly. We utilized the HLA-DQw6 genes of transgenic C57BL/6 mice (DQw6-B6) and their F1 progenies with or without DQw6 genes derived from the matings between DBA/1 mice and HLA-DQw6 hemizygous transgenic DQw6-B6 mice. Mice were immunized with bovine type II collagen (C II) followed by boost immunization 3 weeks later. The development of arthritis was observed and the levels of IgG specific to C II were evaluated on ELISA every week until 10 weeks after the first immunization. The incidence of arthritic mice in DQw6(+)-F1 decreased significantly (P less than 0.05) as compared with that in DQw6(-)-F1 42.9% vs. 80.0%). The incidence of arthritic limbs in DQw6(+)-F1 decreased significantly (P less than 0.01) as compared with that in DQw6(-)-F1 (30.2% vs. 58.3%). The level of IgG specific to C II on the 5th week in DQw6(+)-F1 decreased significantly (P less than 0.01) as compared with that in DQw6(-)-F1 (218.6 +/- 39.2 micrograms/ml, mean +/- SEM, n = 21 vs. 431.3 +/- 58.8 micrograms/ml, mean +/- SEM, n = 20). Thus these results suggested that HLA-DQw6 genes suppressed the development of CIA most likely due to the reduction of the production of IgG specific to type II collagen.
Affiliation
Department of Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka.
External References
- PubMed ID:
- 2022363
Comments
Sign in or create a free account to join the conversation.
Sign in to commentBe the first to comment.