In vitro effect of Lenzites betulinus mushroom against therapy-induced DNA damage in peripheral blood lymphocytes of patients with acute coronary syndrome.
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Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Acute coronary syndrome (ACS) represents a group of diseases that are the result of reduced blood flow to the heart. There are natural products, based on mushrooms, used traditionally in the treatment of cardiovascular diseases. AIM OF THE STUDY: Assessment of the potential protective effect of L. betulinus mushroom against therapy-induced DNA damage in lymphocytes of patients with ACS in relation to the phytochemical properties of the mushroom. MATERIALS AND METHODS: The study included 30 ACS patients and 30 healthy controls. The genotoxic potential of methanol and ethanol extract of L. betulinus was evaluated using the comet assay. The contents of minerals were determined by inductively coupled plasma optical emission spectrometry. Determination of sugars and organic acids was performed using a DIONEX ICS 3000 DP liquid chromatography system. Analysis of fatty acids was performed at Focus GC coupled with PolarisQ mass spectrometer. The total phenolic and flavonoid contents in the mushroom extracts were measured using spectrophotometric methods. The qualitative and quantitative content of polyphenolic compounds was investigated by the UHPLC-DADMS/MS method. RESULTS: The comet assay showed that both mushroom extracts did not increase the level of DNA damage in the lymphocytes of healthy individuals, while they significantly decreased the %DNA damage and genetic damage index (p
Affiliation
Jovana Tubić Vukajlović
External References
- PubMed ID:
- 39084274
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