n/a
Abstract Title:

[10-gingerol inhibits proliferation of hepatocellular carcinoma HepG2 cells via Src/STAT3 signaling pathway].

Abstract Source:

Nan Fang Yi Ke Da Xue Xue Bao. 2018 Jul 30 ;38(8):1002-1007. PMID: 30187878

Abstract Author(s):

Jianxin Chen, Yifen Wu, Shuji Li, Hongyuan Wu, Libo Li

Article Affiliation:

Jianxin Chen

Abstract:

OBJECTIVE: To study the inhibitory effect of 10-gingerol on the proliferation of hepatocellular carcinoma HepG2 cells and the role of Src/STAT3 signaling pathway in mediating the effect.

METHODS: SYBYL-X2.1 software was used to simulate the interaction between 10-gingerol and Src. HepG2 cells treated with 10-gingerol at 1, 3, 10 orμol/L for 24 h were assessed for cell viability using MTT assay, and EdU staining was used to detect the cell proliferation and calculate the number of positive cells. The expressions of p-Src and p-STAT3 were detected using Western blotting, and the mRNA expressions of the target genes of STAT3 (cyclin D1 and CMCC) were detected using qPCR.

RESULTS: 10-gingerol was capable of forming hydrogen bond with such Src residues as TRY-340, MET-341, MET-314, ASP-404, and ILE-336. MTT assay showed that 10-gingerol at 3 and 10μmol/L significantly lowered the viability of HepG2 cells (<0.001). Treatment with 1, 3, and 10μmol/L 10-gingerol significantly reduces the number of EdU-positive HepG 2 cells (<0.001). Western blotting showed that 10-gingerol at 3 and 10μmol/L significantly decreased the phosphorylation levels of Src and STAT3 in HepG2 cells (<0.01). 10-gingerol at 1, 3, and 10μmol/L significantly decreased the mRNA expressions of cyclin D1 and CMCC as shown by qPCR (<0.01).

CONCLUSIONS: 10-gingerol can dose-dependently inhibit the proliferation of HepG2 cells and suppress the activation of Src and STAT3.

Study Type : In Vitro Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.