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Article Publish Status: FREE
Abstract Title:

10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales.

Abstract Source:

Front Cell Dev Biol. 2021 ;9:588093. Epub 2021 Mar 3. PMID: 33748100

Abstract Author(s):

Liqing Zang, Kazuhiro Kagotani, Hiroko Nakayama, Jacky Bhagat, Yuki Fujimoto, Akihito Hayashi, Ryoji Sono, Hirotaka Katsuzaki, Norihiro Nishimura, Yasuhito Shimada

Article Affiliation:

Liqing Zang

Abstract:

Osteoporosis is the most common aging-associated bone disease and is caused by hyperactivation of osteoclastic activity. We previously reported that the hexane extract of ginger rhizome [ginger hexane extract (GHE)] could suppress receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells. However, the anti-osteoclastic components in GHE have not yet been identified. In this study, we separated GHE into several fractions using silica gel column chromatography and evaluated their effects on osteoclastogenesis using a RAW264.7 cell osteoclast differentiation assay () and the zebrafish scale model of osteoporosis (). We identified that the fractions containing 10-gingerol suppressed osteoclastogenesis in RAW264.7 cells detected by tartrate-resistant acid phosphatase (TRAP) staining. In zebrafish, GHE and 10-gingerol suppressed osteoclastogenesis in prednisolone-induced osteoporosis regenerated scales to promote normal regeneration. Gene expression analysis revealed that 10-gingerol suppressed osteoclast markers in RAW264.7 cells [osteoclast-associated immunoglobulin-like receptor, dendrocyte-expressed seven transmembrane protein, and matrix metallopeptidase-9 ()] and zebrafish scales [osteoclast-specific cathepsin K (CTSK),, and]. Interestingly, nuclear factor of activated T-cells cytoplasmic 1, a master transcription regulator of osteoclast differentiation upstream of the osteoclastic activators, was downregulated in zebrafish scales but showed no alteration in RAW264.7 cells. In addition, 10-gingerol inhibited CTSK activity under cell-free conditions. This is the first study, to our knowledge, that has found that 10-gingerol in GHE could suppress osteoclastic activity in bothandconditions.

Study Type : Animal Study, In Vitro Study
Additional Links
Pharmacological Actions : Osteoprotective : CK(1840) : AC(616)

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