Article Publish Status: FREE
Abstract Title:

Addition of bromelain and acetylcysteine to gemcitabine potentiates tumor inhibitionin human colon cancer cell line LS174T.

Abstract Source:

Am J Cancer Res. 2021 ;11(5):2252-2263. Epub 2021 May 15. PMID: 34094682

Abstract Author(s):

Ahmed H Mekkawy, Krishna Pillai, Samina Badar, Javed Akhter, Kevin Ke, Sarah J Valle, David L Morris

Article Affiliation:

Ahmed H Mekkawy


The combinations of Bromelain and Acetylcysteine (BromAc) with cytotoxics such as Gemcitabine, 5-Fluorouracil or Oxaliplatin have shown a dramatic reduction in IC50 values in a variety of cancers, including colon cancer, suggesting the possibility of effective treatment without undesired side effects. In the current study, we investigated whether a similar effect is presentusing the colorectal cell line LS174T. Animals after acclimatization were randomized and allocated equally in the groups for the different studies (safety, dose-escalation, and efficacy). Drugs were delivered by the intraperitoneal route and animals were monitored for wellbeing. Separately, an efficacy study was conducted with intraperitoneal drug delivery after intraperitoneal tumor induction. At the termination of the experiment, tumors and other tissues were collected for evaluation. BromAcwas safe when delivered intraperitoneally in a rat model at the concentrations used. Subsequent investigations of these adjuvants in combination with Gemcitabine, Oxaliplatin, and 5-Fluorouracil in mice were also proven to be safe. Preliminary efficacy studies with Oxaliplatin and 5-Fluorouracil on tumor growth (LS174T) were negative. Gemcitabine was assessed with BromAcshowing an almost 71% tumor inhibition compared to controls. Thisstudy indicates that Gemcitabine at 2 mg/kg in combination with BromAc3 mg/300 mg/Kg was effective and safe, supporting its potential for future clinical application.

Study Type : Animal Study, In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.