Article Publish Status: FREE
Abstract Title:

Micronuclei Assessment of The Radioprotective Effects of Melatonin and Vitamin C in Human Lymphocytes.

Abstract Source:

Cell J. 2016 ;18(1):46-51. Epub 2016 Apr 4. PMID: 27054118

Abstract Author(s):

Aram Rostami, Seyed Akbar Moosavi, Hassan Dianat Moghadam, Eftekhar Rajab Bolookat

Article Affiliation:

Aram Rostami


OBJECTIVE: Critical macromolecules such as DNA maybe damaged by free radicals that are generated from the interaction of ionizing radiation with biological systems. Melatonin and vitamin C have been shown to be direct free radical scavengers. The aim of this study was to investigate the in vivo/in vitro radioprotective effects of melatonin and vitamin C separately and combined against genotoxicity induced by 6 MV x-ray irradiation in human cultured blood lymphocytes.

MATERIALS AND METHODS: In this experimental study, fifteen volunteers were divided into three groups of melatonin, vitamin C and melatonin plus vitamin C treatment. Peripheral blood samples were collected from each group before, and 1, 2 and 3 hours after melatonin and vitamin C administration (separately and combined). The blood samples were then irradiated with 200 cGy of 6 MV x-ray. In order to characterize chromosomal aberrations, the lymphocyte samples were cultured with mitogenic stimulus on cytokinesisblocked binucleated cells.

RESULTS: The samples collected 1hour after melatonin and vitamin C (separately and combined) ingestion exhibited a significant decrease in the incidence of micronuclei compared with their control group (P<0.05). The maximum synergic protection and reduction in frequency of micronuclei (57%) was observed 1 hour after vitamin C and melatonin administration combined.

CONCLUSION: We conclude that simultaneous administration of melatonin and vitamin C as radioprotector substances before irradiation may reduce genotoxicity caused by x-ray irradiation.

Study Type : Animal Study

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