Article Publish Status: FREE
Abstract Title:

Cyanidin-3-O-Glucoside Modulates the In Vitro Inflammatory Crosstalk between Intestinal Epithelial and Endothelial Cells.

Abstract Source:

Mediators Inflamm. 2017 ;2017:3454023. Epub 2017 Mar 8. PMID: 28373746

Abstract Author(s):

Daniela Ferrari, Francesco Cimino, Deborah Fratantonio, Maria Sofia Molonia, Romina Bashllari, Rossana Busà, Antonella Saija, Antonio Speciale

Article Affiliation:

Daniela Ferrari


Intestinal epithelium represents a protective physical barrier and actively contributes to the mucosal immune system. Polarized basolateral intestinal secretion of inflammatory mediators, followed by activation of NF-B signaling and inflammatory pathways in endothelial cells, efficiently triggers extravasation of neutrophils from the vasculature, therefore contributing to the development and maintenance of intestinal inflammation. Proper regulation of NF-B activation at the epithelial interface is crucial for the maintenance of physiological tissue homeostasis. Many papers reported that anthocyanins, a group of compounds belonging to flavonoids, possess anti-inflammatory effects and modulate NF-B activity. In this study, by using a coculture in vitro system, we aimed to evaluate the effects of TNF--stimulated intestinal cells on endothelial cells activation, as well as the protective effects of cyanidin-3-glucoside (C3G). In this model, TNF-induced nuclear translocation of NF-B and TNF-and IL-8 gene expression in Caco-2 cells, whereas C3G pretreatment dose-dependently reduced these effects. Furthermore, TNF--stimulated Caco-2 cells induced endothelial cells activation with increased E-selectin and VCAM-1 mRNA, leukocyte adhesion, and NF-B levels in HUVECs, which were inhibited by C3G. We demonstrated that selective inhibition of the NF-B pathway in epithelial cells represents the main mechanism by which C3G exerts these protective effects. Thus, anthocyanins could contribute to the management of chronic gut inflammatory diseases.

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