Anti-infective potential of hydroalcoholic extract of peel against gram-negative bacterial pathogens.
F1000Res. 2019 ;8:70. Epub 2019 Jan 18. PMID: 30828441
extracts have been prescribed in traditional medicine for management of a variety of disease conditions including microbial infections. Generation of scientific evidence for validation ofpeel extract's anti-pathogenic efficacy is required.Hydroalcoholic extract ofpeel (PGPE), prepared by microwave assisted extraction method was evaluated for its quorum-modulatory potential against two different human-pathogenic bacteria viz.and.This extract was able to modulateproduction of quorum sensing-regulated pigments in both these test bacteria at≥5 μg/ml. Virulence traits oflike haemolytic activity, and biofilm formation were negatively affected by the test extract, and it also mademore susceptible to lysis by human serum. Antibiotic susceptibility of both test bacteria was modulated owing to pre-treatment with PGPE. Exposure of these test pathogens to PGPE (≥0.5 μg/ml) effectively reduced their virulence towards the nematode. Repeated subculturing ofon PGPE-supplemented growth medium did not induce resistance to PGPE in this notorious pathogen, and this extract was also found to exert a post-extract effect onIndividual constituent phytocompounds of PGPE were found to be less efficacious than the whole extract. PGPE seemed to interfere with themachinery ofand. PGPE also exhibited notable prebiotic potential by promoting growth of probiotic strains-andat≤50 μg/ml.This study indicates PGPE to be an effective antipathogenic and prebiotic preparation, and validates its therapeutic use mentioned in traditional medicine. This study also emphasizes the need for testing any bioactive extract at broadest possible concentration range, particularly, so that an accurate picture of dose-response relationship can emerge.