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Abstract Title:

Anti-inflammatory activity of ethanol extract derived from Phaseolus angularis beans.

Abstract Source:

J Ethnopharmacol. 2011 Oct 11 ;137(3):1197-206. Epub 2011 Aug 2. PMID: 21821108

Abstract Author(s):

Tao Yu, Hyo Min Ahn, Ting Shen, Keejung Yoon, Hyun-Jae Jang, Yong Jin Lee, Hyun Mo Yang, Jae Hun Kim, Changhyuk Kim, Moon Hi Han, Sang-Hun Cha, Tae Wong Kim, Sun Young Kim, Jaehwi Lee, Jae Youl Cho

Article Affiliation:

Tao Yu

Abstract:

ETHNOPHARMACOLOGICAL SIGNIFICANCE: Phaseolus angularis Wight (adzuki bean) is an ethnopharmacologically well-known folk medicine that is prescribed for infection, edema, and inflammation of the joints, appendix, kidney and bladder in Korea, China and Japan.

AIM OF STUDY: The anti-inflammatory effect of this plant and its associated molecular mechanisms will be investigated.

MATERIALS AND METHODS: The immunomodulatory activity of Phaseolus angularis ethanol extract (Pa-EE) in toll like receptor (TLR)-activated macrophages induced by ligands such as lipopolysaccharide (LPS), Poly (I:C), and pam3CSK was investigated by assessing nitric oxide (NO) and prostaglandin (PG)E(2) levels. To identify which transcription factors such as nuclear factor (NF)-κB and their signaling enzymes can be targeted to Pa-EE, biochemical approaches including reporter gene assays, immunoprecipitation, kinase assays, and immunoblot analyses were also employed. Finally, whether Pa-EE was orally available, ethanol (EtOH)/hydrochloric acid (HCl)-induced gastritis modelin mice was used.

RESULTS: Pa-EE dose-dependently suppressed the release of PGE(2) and NO in LPS-, Poly(I:C)-, and pam3CSK-activated macrophages. Pa-EE strongly down-regulated LPS-induced mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Interestingly, Pa-EE markedly inhibited NF-κB, activator protein (AP)-1, and cAMP response element binding protein (CREB) activation; further, according to direct kinase assays and immunoblot analyses, Pa-EE blocked the activation of the upstream signaling molecules spleen tyrosine kinase (Syk), p38, and transforming growth factor β-activated kinase 1 (TAK1). Finally, orally administered Pa-EE clearly ameliorated EtOH/HCl-induced gastritis in mice.

CONCLUSION: Our results suggest that Pa-EE can be further developed as a promising anti-inflammatory remedy because it targets multiple inflammatory signaling enzymes and transcription factors.

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