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Abstract Title:

Antivirulence Activity of a Dietary Phytochemical: Hibiscus Acid Isolated fromL. Reduces the Virulence ofin a Mouse Infection Model.

Abstract Source:

J Med Food. 2021 Mar 22. Epub 2021 Mar 22. PMID: 33751918

Abstract Author(s):

Humberto Cortes-López, Javier Castro-Rosas, Rodolfo García-Contreras, José Salud Rodríguez-Zavala, Bertha González-Pedrajo, Miguel Díaz-Guerrero, Javier Hernández-Morales, Naybi Muñoz-Cazares, Marcos Soto-Hernández, Lucero Del Mar Ruíz-Posadas, Israel Castillo-Juárez

Article Affiliation:

Humberto Cortes-López

Abstract:

L. () calyxes, rich in organic acids, are included in diets in different countries. In recent years, some phytochemicals have been shown to reduce bacterial virulence at sublethal concentrations by interfering with quorum sensing (QS) systems. Therefore, in this study the antivirulence properties ofcalyxes and two-lactones (hibiscus acid [HA] and its methyl ester) inwere analyzed. Acetone and methanol extracts ofshowed anti-QS activity by inhibiting violacein production (60% to 80% with 250 g/mL). In molecular docking analysis, the-lactones registered a good binding score, which suggests strong interaction with the active site of LasR protein. To verify their effect, they were isolated fromand evaluated in six QS-regulated phenotypes, as well as in ExoU toxin that is released by the type III secretion system (T3SS). At 500 g/mL they reduced alkaline protease (29-52%) and elastase (15-37%) activity, biofilm formation (∼75%), and swarming (50%), but there was no effect on pyocyanin production, hemolytic activity, or type III secretion. In a mouse abscess/necrosis model, HA at sublethal concentrations (15 and 31.2 g/mL) affected infection establishment and prevented damage and systemic spread. In conclusion, HA is the first molecule identified with antivirulence properties inwith the potential to prevent infections caused by.

Study Type : Animal Study, In Vitro Study

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