Antibacterial and antibiofilm effect of cell-free supernatant of Lactobacillus brevis KCCM 202399 against Streptococcus mutans KCTC 5458. - GreenMedInfo Summary

This study aims to determine the antibiofilm effect of cell-free supernatant (CFS) ofstrains againststrains. To study the antibiofilm mechanism againststrains, antibacterial effects, cell surface properties (auto-aggregation and cell surface hydrophobicity), exopolysaccharide (EPS) production, and morphological changes were examined. The antibiofilm effect ofKCCM 202399 CFS as morphological changes were evaluated by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), compared with the control treatment. Among thestrains,KCCM 202399 showed the highest antibiofilm effect onKCTC 5458. The antibacterial effect ofKCCM 202399 againstKCTC 5458 was investigated using the deferred method (16.00 mm). The minimum inhibitory concentration ofKCCM 202399 againstKCTC 5458 was 25.00%. Compared with the control treatment,KCCM 202399 CFS inhibited the bacterial adhesion ofKCTC 5458 by decreasing auto-aggregation, cell surface hydrophobicity, and EPS production (45.91%, 40.51%, and 67.44%, respectively). L. brevis KCCM 202399 CFS inhibited and eradicated theKCTC 5458 biofilm. Therefore, these results suggest thatKCCM 202399 CFS may be used to develop oral health in the probiotic industry.