Antibacterial and antioxidant effects ofL. extract and its fractions.
J Tradit Complement Med. 2019 Oct ;9(4):383-392. Epub 2018 Oct 9. PMID: 31453135
Guilherme Pires Amaral
The production of reactive species over physiological levels associated to pathogenic bacteria could represent a high risk for many diseases. TheL. is used around the world due its pharmacological proprieties. So, in this study our aim is to test for the first time ifL. extract (eeRo) and its fractions (DCM, EA, ButOH) could have better or similar antioxidant action to standars and among themselvesorin brain, stomach and liver of rats. Moreover, we intend to clarify their possible effects on pathogenic bacteria. The eeRo was obtained from the dried leaves subjected to an alcoholic extraction and fractioned. The quantification of the constituents of eeRo and fractions were done by HPLC. The antioxidant proprieties ofwas analyzed by DPPHradical scavenging, total antioxidant, dichlorofluorescein, lipid peroxidation and sodium nitroprusside -induced lipid peroxidation assays. The Minimum inhibitory concentrations ofL. were tested with standard strains of danger bacteria. The eeRo, DCM, EA had significant total antioxidant and DPPHradical scavenging activities. The DCM and eeRo got significant effects against basal levels of reactive species in liver, stomach and brain. The eeRo and DCM protected the liver and brain against lipid peroxidation. The eeRo, DCM, EA and ButOH had inhibitory effect in the Gram-positive and Gram-negative bacteria. In general way, the DCM and eeRo had the best antioxidant and antibacterial effects among all tested fractions.