Apigenin significantly inhibits the proliferation, migration, and invasion of Tca8113 cells. - GreenMedInfo Summary
Apigenin Inhibits Proliferation, Migration, and the Invasion of human tongue carcinoma Tca8113 Cells through Regulating the MAPK Signaling Pathways.
Curr Mol Med. 2020 Oct 22. Epub 2020 Oct 22. PMID: 33092506
OBJECTIVE: This study aimed to examine the effects of apigenin (API) on the proliferation, migration, and invasion of human tongue squamous cell carcinoma Tca8113 cells and explore its probable mechanisms.
METHODS: After treating Tca8113 cells with API, the cell proliferation, migration, and invasivecapacities were identified by te-trazolium salt colorimetry(MTT) assay, cell scratch test, and Transwell chamber test. Cellular immunofluorescence staining was used to localize mitogen-activated protein kinase 1 (MAPK1) and extracellular regulated protein kinase (ERK) 1/2 proteins. Western blot was used to detect the variations of the related protein expression levels.
RESULTS: ① Through the MTT assay, API significantly inhibited cell proliferation (P<0.01).②In the cell scratch test, the dis-tance of lateral migration after the API treatment was significantly shorter compared to the control group (P<0.01).③The inva-sion rate in the lower chamber of the Transwell chamber was lower in the API group (P<0.01).④ Cellular immunofluores-cence staining presented that the total-MEKK1 was localized in the cytoplasm, p-MEKK1 was localized in the nuclear mem-brane and cytoplasm, and p-ERK1/2 was localized in the cytoplasm and nucleus. ⑤ After API was applied to cells, the expres-sions of p-MEKK1 and p-ERK1/2proteins significantly reduced (P<0.01).
CONCLUSION: Apigenin(API) significantly inhibits the proliferation, migration, and invasion of Tca8113 cells and its mecha-nism may be associatedwith the MAPK signaling pathway.