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Abstract Title:

Extract Fermented byEJ2014 Modulates Immune Response in Mice.

Abstract Source:

Antioxidants (Basel). 2021 Aug 11 ;10(8). Epub 2021 Aug 11. PMID: 34439524

Abstract Author(s):

Md Sekendar Ali, Eon-Bee Lee, Seung-Jin Lee, Sam-Pin Lee, Naila Boby, Kyoungho Suk, Biruk Tesfaye Birhanu, Seung-Chun Park

Article Affiliation:

Md Sekendar Ali

Abstract:

The present study aimed to assess the immunomodulatory effects of fermentedextract (FAME) on RAW 264.7 cells and BALB/c mice.fruit was fermented withEJ2014 by adding yeast extract and monosodium glutamate for 9 days at 30°C to produce γ-aminobutyric acid (GABA). After fermentation, significant GABA production was noted, along with minerals, polyphenols, and flavonoids (<0.05). The polyphenol content was confirmed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. RAW 264.7 cells were stimulated with lipopolysaccharide (LPS, 1μg/mL) in the presence or absence of FAME, and proinflammatory cytokine contents were measured by qPCR. In the in vivo experiment, female BALB/c mice were administered 125, 250, and 500 mg/kg of FAME for 21 days. FAME treatment increased neutrophil migration and phagocytosis (<0.05). It also increased splenocyte proliferation, CD4and CD8T-cell expression, and lymphocyte proliferation. Furthermore, it increased IFN-γ, IL-2, and IL-4 cytokine levels in a dose-dependent manner (<0.05). However, it decreased TNF-α and IL-6 levels (<0.05). These results indicate that FAME fortified with GABA including bioactive compounds exerts anti-inflammatory effects by inhibiting proinflammatory cytokines in RAW 264.7 cells and modulates immune response in mice. Thus, FAME could be a potential therapeutic agent for inflammatory disorders.

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