The Protective Effect of Different Extracts of ThreeSpecies against HO-Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells.
Pharmacognosy Res. 2018 Jan-Mar;10(1):64-71. PMID: 29568190
Background: Oxidative stress causes cell damage and is involved in many neurological diseases. The antioxidant properties of plant materials for the maintenance of health and protecting against different diseases stimulated scientist to investigate different herbs. Differentspecies have exhibited antioxidant activity. This study aims to investigate whether differentspecies could protect the PC12 cells against oxidative stress mediated by HO.
Methods: For this purpose, different extracts of threespecies (,, and) were prepared using petroleum ether, dichloromethane, ethyl acetate, ethanol, and Water: Ethanol mixture (1:1 volume ratio). The protective effect of the prepared extracts against HO-induced cytotoxicity and reactive oxygen species production were compared. The effect of treatment of PC12 cells with different extracts on total glutathione (GSH) level, caspase-3 activity, and mitochondrial membrane potential were also compared.
Results: Theextracts could not rescue the PC12 cells from oxidative stress consequences. Theandextracts were found potent in suppressing the toxicity and apoptosis of PC12 cells mediated by HOand significantly antagonized the HO-induced GSH depletion. The hydroethanolic and ethyl acetate extracts ofand the petroleum ether and hydroethanolic extracts ofmore efficiently suppressed cytotoxicity and loss of GSH caused by HO.
Conclusion: This study shows the protective effects ofextracts on PC12 cell line and suggested that these species could be also considered as promising neuroprotective agents in treatment of different neurodegenerative diseases.
SUMMARY: andextracts were found to potentially exert neuroprotective effect on PC12 cells. The results exhibited that the cytoprotective potential and anti-apoptotic mechanism of these species is not the same for different extracts, and suggested that based on the type of species and the type of solvents used in extraction, both intrinsic and extrinsic pathways could be included in the anti-apoptotic mechanism of these species.GSH: Glutathion. ROS: Reactive Oxygen Species. GSSG: Glutathione disulfide. DCF-DA:2',7'-Dichlorofluorescin diacetate. FBS: Fetal Bovin Serum. MMP: Mitochondrial Membrane Potential. H-Et: Hydro-ethanolic. DCM: Dichloromethane. PE: Petroleum Ether. Et: Etanolic. EA: Ethyl Acetate.