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Article Publish Status: FREE
Abstract Title:

Ascorbic acid modulates the structure of thevirulence factor pyocyanin and ascorbic acid-furanone-30 combination facilitate biofilm disruption.

Abstract Source:

Front Microbiol. 2023 ;14:1166607. Epub 2023 Jul 13. PMID: 37520362

Abstract Author(s):

Theerthankar Das, Biswanath Das, Brandon Clark Young, Vina Aldilla, Shekh Sabir, Basmah Almohaywi, Mark Willcox, Mike Manefield, Naresh Kumar

Article Affiliation:

Theerthankar Das

Abstract:

The production of pyocyanin byincreases its virulence, fitness and biofilm formation. Pyocyanin is also a redox molecule and we hypothesize that ascorbic acid being an antioxidant will interact with pyocyanin. The main objective of this study was to investigate the potential interaction of ascorbic acid with pyocyanin, and also to investigate the impact of ascorbic acid in combination with Furanone-30 on quorum sensing and biofilm formation of. When incubated with ascorbic acid, hyperchromic and hypsochromic shifts in pyocyanin absorbance peaks at 385 nm and 695 nm were observed. In the presence of dehydroascorbic acid and citric acid, these shifts were absent, indicating that the intrinsic antioxidant property of ascorbic acid was probably essential in binding to pyocyanin. NMR spectroscopy showed shifts inH NMR pyocyanin peaks between 8.2 to 5.8 ppm when incubated in the presence of ascorbic acid. Density Functional Theory (DFT) supported potential interactions between the -CHOH or -OH moieties of ascorbic acid with the -C=O moiety of pyocyanin. The pyocyanin-ascorbic acid complex impaired pyocyanin binding to DNA. Ascorbic acid combined with furanone-30 elevated quorum-sensing inhibition in, which was directly associated with significantly reducedvirulence, adhesion, aggregation and biofilm formation and enhanced antibiotic-mediated bacterial killing. This study demonstrated that the antioxidant ascorbic acid directly binds to pyocyanin, modulates its structure and results in disruption of biofilm formation and associated tolerance to antibiotics.

Study Type : In Vitro Study

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