Betulinic acid exerts anti-inflammatory activity in human periodontal ligament cells stimulated with lipopolysaccharide or high glucose. - GreenMedInfo Summary
Betulinic acid exerts anti-inflammatory activity in human periodontal ligament cells stimulated with lipopolysaccharide and/or high glucose.
Endocr Metab Immune Disord Drug Targets. 2022 May 9. Epub 2022 May 9. PMID: 35538811
Ping Hu
Background: Diabetic patients have weakened periodontal ligament and an increased risk of periodontitis due to uncontrolled glycemia. Betulinic acid (BA), a hypoglycemic drug, has anti-inflammatory activities. Objectives: The current study was to explore the protective effect of BA on the inflammation inhuman periodontal ligament cells (PDLCs) stimulated with lipopolysaccharide (LPS) and/or high glucose (HG) status and its mechanisms of action. Methods: Human PDLCs were exposed to LPS and/or HG, with or without BA intervention. The production of nitrite oxide (NO) and prostaglandin E2 (PGE2) werequantified by Griess reaction and enzyme-linked immunosorbent assay, respectively. Immunoblotting analyses were employed to detect the expression of inducible nitric oxide synthase (iNOS) and the cyclooxygenase-2 (COX-2), as well as the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa-B (NF-κB) in human PDLCs. Results: The increased production of iNOS/NO and COX-2/PGE2 and significantly increased phosphorylated levels of IκBα, JNK and p38 can be detected in human PDLCs with LPS and/or HG situation, while increased phosphorylated ERK can be seen in cells under only LPS condition. Furthermore, the non-toxic concentration of BA (10 µM) prevented NF-κB and MAPKs activation and partly but significantly reversed the induction of COX-2/ PGE2 and iNOS/NO in human PDLCs with LPS and/or HG loaded. Conclusion: BA was proved for the first time to protect human PDLCs from the LPS-induced and/or HG-induced inflammation, which works through the mechanism involving the action of MAPKs and NF-κB. signaling pathways. Thus, BA could be used to alleviate diabetic complications of periodontitis.