Bioactivity-Guided Identification of Anti-Adipogenic Isothiocyanates in the Moringa () Seed and Investigation of the Structure-Activity Relationship.
Molecules. 2020 May 28 ;25(11). Epub 2020 May 28. PMID: 32481514
Due to the side effects of obesity medications, many studies have focused on the natural products used in the daily diet to control weight. Moringa seed pods and leaves are widely used as vegetables or diet supplements due to the high nutrition value. However, no bioactivity-guided anti-adipogenic study was previously conducted. Therefore, a preadipocyte cell line was adopted as the bioactivity assay to identify the anti-adipogenic compounds in the peeled Moringa seed. Two known sulphur-containing compounds (and) were isolated and identified. Compound, 4-(α-l-rhamnosyloxy) benzyl isothiocyanateshowed a great anti-adipogneic effect with an ICvalue of 9.2μg/mL. The isothiocyanate (ITC) group in compoundcould be responsible for the inhibitory activity. In addition, a series of compounds with the ITC group were used to further investigate the structure-activity relationship, indicating foods containing ITC derivatives have the potential of being used to control weight.