Bisphenol S impaired in vitro ovine early developmental oocyte competence. - GreenMedInfo Summary
Bisphenol S Impaired In Vitro Ovine Early Developmental Oocyte Competence.
Int J Mol Sci. 2020 Feb 12 ;21(4). Epub 2020 Feb 12. PMID: 32059612
INTRODUCTION: Bisphenol A (BPA) is a widespread compound in the plastic industry that is especially used to produce baby bottles, food packaging and metal cans. BPA, an endocrine disruptor, leads to alterations in reproductive function and therefore has been banned from the food industry. Unregulated BPA analogues, particularly Bisphenol S (BPS), have emerged and are now used in the plastic industry. Thus, this study aimed to examine the acute effects of low and environmental doses of BPS on ewe oocyte quality and developmental competence, and its mechanism of action, during in vitro maturation.
METHODS: Ewe cumulus-oocyte complexes underwent in vitro maturation in the presence or absence of BPS (1 nM, 10 nM, 100 nM, 1µM or 10 µM). Oocytes were then subjected to in vitro fertilisation and development.
RESULTS: 1µM BPS induced a 12.7% decrease in the cleavage rate (= 0.004) and a 42.6% decrease in the blastocyst rate (= 0.017) compared to control. The blastocyst rate reduction was also observed with 10 nM BPS. Furthermore, 10µM BPS reduced the oocyte maturation rate, and 1 µM BPS decreased cumulus cell progesterone secretion. PR and AMH gene expression were reduced in cumulus cells. BPS induced a 5-fold increase in MAPK 3/1 activation (= 0.04).
CONCLUSIONS: BPS impaired ewe oocyte developmental competence. The data suggest that BPS might not be a safe BPA analogue. Further studies are required to elucidate its detailed mechanism of action.