Abstract Title:

Isolation of cyanidin 3-glucoside from blue honeysuckle fruits by high-speed counter-current chromatography.

Abstract Source:

Food Chem. 2014 ;152:386-90. Epub 2013 Nov 22. PMID: 24444952

Abstract Author(s):

Liang Chen, Xiulan Xin, Rong Lan, Qipeng Yuan, Xiaojie Wang, Ye Li

Article Affiliation:

Liang Chen


Blue honeysuckle fruits are rich in anthocyanins with many beneficial effects such as reduction of the risk of cardiovascular diseases, diabetes and cancers. High-speed counter-current chromatography (HSCCC) was used for the separation of anthocyanin on a preparative scale from blue honeysuckle fruit crude extract with a biphasic solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (2:2:1:5:0.01, v/v) for the first time in this paper. Each injection of 100 mg crude extract yielded 22.8 mg of cyanidin 3-glucoside (C3G) at 98.1% purity. The compound was identified by means of electro-spray ionisation mass (ESI/MS) and (1)H and (13)C nuclear magnetic resonance (NMR) spectra.

Study Type : Plant Study

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