Abstract Title:

Blueberry Supplementation Influences the Gut Microbiota, Inflammation, and Insulin Resistance in High-Fat-Diet-Fed Rats.

Abstract Source:

J Nutr. 2018 Feb 1 ;148(2):209-219. PMID: 29490092

Abstract Author(s):

Sunhye Lee, Katherine I Keirsey, Rebecca Kirkland, Zachary I Grunewald, Joan G Fischer, Claire B de La Serre

Article Affiliation:

Sunhye Lee


Background: Gut microbiota dysbiosis has been linked to obesity-associated chronic inflammation. Microbiota manipulation may therefore affect obesity-related comorbidities. Blueberries are rich in anthocyanins, which have anti-inflammatory properties and may alter the gut microbiota.

Objective: We hypothesized that blueberry supplementation would alter the gut microbiota, reduce systemic inflammation, and improve insulin resistance in high-fat (HF)-diet-fed rats.

Methods: Twenty-four male Wistar rats (260-270 g; n = 8/group) were fed low-fat (LF; 10% fat), HF (45% fat), or HF with 10% by weight blueberry powder (HF_BB) diets for 8 wk. LF rats were fed ad libitum, whereas HF and HF_BB rats were pair-fed with diets matched for fiber and sugar contents. Glucose tolerance, microbiota composition (16S ribosomal RNA sequencing), intestinal integrity [villus height, gene expression of mucin 2 (Muc2) andβ-defensin 2 (Defb2)], and inflammation (gene expression of proinflammatory cytokines) were assessed.

Results: Blueberry altered microbiota composition with an increase in Gammaproteobacteria abundance (P<0.001) compared with LF and HF rats. HF feeding led to an∼15% decrease in ileal villus height compared with LF rats (P<0.05), which was restored by blueberry supplementation. Ileal gene expression of Muc2 was∼150% higher in HF_BB rats compared with HF rats (P<0.05), with expression in the LF group not being different from that in either the HF or HF_BB groups. Tumor necrosis factorα (Tnfa) and interleukin 1β (Il1b) gene expression in visceral fat was increased by HF feeding when compared with the LF group (by 300% and 500%, respectively; P<0.05) and normalized by blueberry supplementation. Finally, blueberry improved markers of insulin sensitivity. Hepatic insulin receptor substrate 1 (IRS1) phosphorylation at serine 307:IRS1 ratio was∼35% higher in HF rats compared with LF rats (P<0.05) and HF_BB rats.

Conclusion: In HF-diet-fed male rats, blueberry supplementation led to compositional changes in the gut microbiota associated with improvements in systemic inflammation and insulin signaling.

Study Type : Animal Study

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