Abstract Title:

Botulinum Toxin Complex Increases Paracellular Permeability in Intestinal Epithelial Cells via Activation of p38 Mitogen-Activated Protein Kinase.

Abstract Source:

J Vet Med Sci. 2013 Jul 25. Epub 2013 Jul 25. PMID: 23884081

Abstract Author(s):

Shin-Ichiro Miyashita, Yoshimasa Sagane, Ken Inui, Shintaro Hayashi, Keita Miyata, Tomonori Suzuki, Tohru Ohyama, Toshihiro Watanabe, Koichi Niwa

Article Affiliation:

Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture.


Clostridium botulinum produces a large toxin complex (L-TC) that increases paracellular permeability in intestinal epithelial cells by a mechanism that remains unclear. Here, we show that mitogen-activated protein kinases (MAPKs) are involved in this permeability increase. Paracellular permeability was measured by FITC-dextran flux through a monolayer of rat intestinal epithelial IEC-6 cells, and MAPK activation was estimated from western blots. L-TC of C. botulinum serotype D strain 4947 increased paracellular dextran flux and activated extracellular signal-regulated kinase (ERK), p38, but not c-Jun N-terminal kinase (JNK) in IEC-6 cells. The permeability increase induced by L-TC was abrogated by the p38 inhibitor SB203580. These results indicate that L-TC increases paracellular permeability by activating p38, but not JNK and ERK.

Study Type : Bacterial

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Sayer Ji
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