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Abstract Title:

Bovine lactoferrin inhibits alveolar bone destruction in an orthodontic rat model with periodontitis.

Abstract Source:

Ann Anat. 2021 Apr 22 ;237:151744. Epub 2021 Apr 22. PMID: 33895284

Abstract Author(s):

Yuan Chen, Tian Zhou, Hong-Hong Zhang, Na Kang

Article Affiliation:

Yuan Chen

Abstract:

OBJECTIVE: We aimed to evaluate the effect of bovine lactoferrin (bLF) on alveolar bone destruction and remodelling under orthodontic force (OF) in periodontitis-affected rats.

MATERIAL AND METHODS: After establishing the periodontitis-affected rat model with lipopolysaccharides (LPS), the left maxillaryfirst molars were moved orthodontically under a force of 0.2N. Based on saline or bLF gavage, 54 Sprague-Dawley (SD) rats were randomized into 5 groups: A (blank), P1 (LPS+OF+bLF), P2 (LPS+OF+saline), C1 (OF+bLF), and C2 (OF+saline). Animals were evaluated using micro-computed tomography (micro-CT)followed by haematoxylin and eosin (H&E) and tartrate-resistant acid phosphatase (TRAP) staining, and the LF level was determined using ELISA in the gingival crevicular fluid (GCF) of the experimental teeth. Immunohistochemistry helped to detect expression changes in RANKL, OPG and COX-2.

RESULTS: Micro-CT results indicated that compared with group P2, trabecular number (Tb.N) and trabecular thickness (Tb.Th) in group P1 were higher and bone surface/bone volume (BS/BV) was lower on day 14, while trabecular separation (Tb.Sp) decreased significantly on Day 5 and Day 14 after bLF gavage (P<0.05). This was supported by changes in H&E and TRAP staining. bLF down-regulated RANKL level at both timepoints and up-regulated OPG level on Day 14 in periodontitis rats (P<0.05). The significant changes mentioned above were not observed between group C1 and C2 (P>0.05). No significant change in COX-2 levels were observed in any group (P>0.05). The lactoferrin level in GCF increased significantly after bLF gavage (P<0.05).

CONCLUSION: Bovine lactoferrin inhibited LPS-induced bone destruction, but the bone healing effect was independent of orthodontic aseptic inflammatory bone remodelling.

Study Type : Animal Study
Additional Links
Pharmacological Actions : Osteoprotective : CK(971) : AC(351)

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Sayer Ji
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