Brazilian red propolis extract enhances expression of antioxidant enzyme genesand.
Biosci Biotechnol Biochem. 2020 Sep ;84(9):1820-1830. Epub 2020 Jun 3. PMID: 32490727
Brazilian red propolis reportedly has reactive oxygen species (ROS) scavenging effects, but the cellular mechanisms remain unclear. In the present study, the effects of an ethanol extract of Brazilian red propolis (EERP) on the Nrf2-ARE intracellular antioxidant pathway were examinedand. EERP and its constituents transactivated the reporter gene through the ARE sequence and enhanced the expression of Nrf2-regulated genes in HEK293 cells. It also increased Nrf2 protein in the nucleus, which was partially inhibited by kinase inhibitors. Furthermore, EERP suppressed ROS generation and cytotoxicity induced by-butyl hydroperoxide., orally administered EERP increased the expression of Nrf2-regulated genes in mice liver. These results suggest that EERP is a potential resource for preventing oxidative stress-related diseases as an Nrf2 inducer.